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Wednesday, 7 December 2016
Designing Primers for Alternative Transcripts #1
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Genes are usually expressed as multiple alternative transcripts as a result of alternative splicing of the pre-mRNA. Pan primers amplify al...
Sunday, 7 August 2016
Ways To Ensure Experimental Consistency #3
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Commercial Kits There are many commercial kits available to streamline and simplify various experimental procedures including DNA/RNA e...
Saturday, 16 July 2016
Before Cloning….
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For using restriction enzymes to digest and ligate genes of interest into plasmid vectors, it is good practice to check if your restriction...
Saturday, 2 July 2016
Calculating the Geometric Mean/Geomean of Housekeeping Genes from Real-Time PCR Data Using Excel
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In real-time PCR, it is not uncommon for multiple housekeeping genes to be used for normalising data. Knowing how to calculate an average f...
Saturday, 11 June 2016
Epigenetics – The nth DNA Base
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Classically, DNA is thought to comprise 4 nucleotide bases: adenine, thymine, cytosine, and guanine (A, T, C, and G). However, the discover...
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